Transcript profiling in the milk of dairy ewes fed extruded linseed

نویسندگان

  • Tommaso Giordani
  • Alberto Vangelisti
  • Giuseppe Conte
  • Andrea Serra
  • Lucia Natali
  • Annamaria Ranieri
  • Marcello Mele
  • Andrea Cavallini
چکیده

The identification of genes regulating milk secretion by mammary glands is a key-step for exploiting changes in milk composition induced by different diet regimens. Linseed supplementation is a reliable feeding strategy to enhance polyunsaturated fatty acid content in milk fat from sheep, increasing milk quality and nutraceutical value. To investigate the molecular bases of diet induced differences in milk composition, we collected milk from dairy ewes at 90 day of lactation and after 3 weeks of diet supplementation with extruded linseed. The milk of dairy ewes contains milk somatic cells mostly derived by mammary glands. After isolating milk somatic cells by centrifugation, RNAs were purified from these cells, and Illumina RNA sequencing was performed to analyze RNA synthesis. Our data provide a resource (available at Gene Expression Omnibus database under GSE89163) to be employed for comparative analyses of gene expression in milk somatic cells in different breeds and different diets, with the long-term aim of developing strategies to improve sheep milk quality.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Effects of forage type and extruded linseed supplementation on methane production and milk fatty acid composition of lactating dairy cows.

Replacing dietary grass silage (GS) with maize silage (MS) and dietary fat supplements may reduce milk concentration of specific saturated fatty acids (SFA) and can reduce methane production by dairy cows. The present study investigated the effect of feeding an extruded linseed supplement on milk fatty acid (FA) composition and methane production of lactating dairy cows, and whether basal forag...

متن کامل

Milk Yield and Blood Metabolite Profile in Late Pregnancy in Lori Ewes Receiving Diets Containing Undegradable Protein Sources

An experiment was conducted to investigate the effects of supplementing Lori ewes with rumen-undegradable protein (RUP) in late pregnancy on their performance and that of their lambs. Milk yield and blood metabolite profile were measured. Forty pregnant ewes were used in a completely randomized design trial with four treatments: 1) a basal diet (control); 2) with a supplement of 5% fishmeal; 3)...

متن کامل

Milk production, milk fatty acid composition, and blood biochemical parameters of Holstein dairy cows fed whole or ground flaxseed instead of extruded soybeans in the first half of lactation

To evaluate the effects of physically processed flaxseed [ground flaxseed (GFS) and whole flaxseed(WFS)] as a replacement for extruded soybeans on feed intake, milk production, milk composition, milk fatty acid (FA) composition and blood biochemical parameters, nine primiparus Holstein dairy lactating cowsaveraging 495 ± 34.5 kg of body weight (BW) and 70 ± 5 days in milk (DIM) were assigned in...

متن کامل

The Challenge of Changing the Fatty Acid Profile of Milk

Feeding dairy cows a ration containing whole flax seeds (linseed), flax (linseed) oil or milled, extruded or micronized linseed has five beneficial effects on the fat profile of cow’s milk: (1) increasing the content of alpha-linolenic acid (ALA); (2) increasing the content of conjugated linoleic acid (CLA); (3) decreasing the omega-6/omega-3 ratio; (4) decreasing the overall saturated fat cont...

متن کامل

Conjugated linolenic acid (CLnA), conjugated linoleic acid (CLA) and other biohydrogenation intermediates in plasma and milk fat of cows fed raw or extruded linseed.

Thirty lactating dairy cows were used in a 3 × 3 Latin-square design to investigate the effects of a raw or extruded blend of linseed and wheat bran (70:30) on plasma and milk fatty-acids (FA). Linseed diets, containing 16.6% linseed blend on a dry-matter basis, decreased milk yield and protein percentage. They decreased the proportions of FA with less than 18 carbons in plasma and milk and res...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره 11  شماره 

صفحات  -

تاریخ انتشار 2017